{"id":208,"date":"2022-03-22T15:42:00","date_gmt":"2022-03-22T19:42:00","guid":{"rendered":"http:\/\/bio.sites.mtu.edu\/lead-remediation\/?p=208"},"modified":"2022-04-09T13:57:05","modified_gmt":"2022-04-09T17:57:05","slug":"phase-2-first-edds-application","status":"publish","type":"post","link":"http:\/\/bio.sites.mtu.edu\/lead-remediation\/2022\/03\/22\/phase-2-first-edds-application\/","title":{"rendered":"Phase 2: First EDDS application"},"content":{"rendered":"\n<p>The first chelating agent application was EDDS at 10 mmol\/kg. Leaching samples were analyzed before and after treatment of EDDS to determine pH, turbidity, and conductivity. Plant and soil samples were analyzed 15 days after treatment of chelating agents. The results showed that the first application of EDDS increased the solubility of lead and lead uptake. The fescue showed a similar result with a slight increase in root and shoot lead concentrations for both Baltimore soil and San Antonio. Overall the result showed that the roots had higher lead concentrations compared to the shoots and that EDDS increased translocation. The total amount of lead was analyzed; vetiver grass lead concentrations were 28.6 to 46.48 mg in San Antonio and 10.28-31.47 mg for Baltimore soil. Fescue lead concentrations were between 1.37-1.88 mg for both soils. Overall, Vetiver, after the first application of EDDS, was more effective at uptaking lead compared to fescue (Datta, Sarkar, and Andra). <\/p>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-default is-cropped wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"573\" height=\"496\" data-id=\"233\" src=\"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.35.34-PM.png\" alt=\"\" class=\"wp-image-233\" srcset=\"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.35.34-PM.png 573w, http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.35.34-PM-300x260.png 300w\" sizes=\"auto, (max-width: 573px) 100vw, 573px\" \/><\/figure>\n<\/figure>\n\n\n\n<p><br> The leach samples were collected several times throughout the experiment and analyzed. After the EDDS application, both soils showed an increase in the solubility of lead compared to before application. Overall the result shows that fescue leached more lead than Vetiver (Datta, Sarkar, and Andra). <br> Soils samples were collected before and after EDDS application. For the Baltimore soils, lead from the vetiver grass was between 1223-1830 mg\/kg and 1246-1870 mg\/kg lead in fescue. For the San Antonio soils, the lead from the vetiver grass was 972-2362 mg\/kg and 1012-2388 mg\/kg from the fescue. After the EDDS application, Baltimore soils decreased Vetiver from 1170-1753 and fescue 1232 -1865 mg\/kg. For the San Antonio soils, both Vetiver and fescue showed a decrease with 945-2300 mg\/kg and 997-2375 mg\/kg. Overall, EDDS application resulted in a decrease in the soluble lead, and Vetiver had less lead concentrations compared to fescue (Datta, Sarkar, and Andra). <br> Vetiver and fescue grasses were harvested after the first EDDS application and set for composting. Lead toxicity from the composting grasses was analyzed. The results showed that small amounts of lead leached from the compost but values were lower than the USEPA standards. Overall it showed that cut grass is not hazardous and can be disposed of normally (Datta, Sarkar, and Andra). <\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:100%\">\n<figure class=\"wp-block-gallery has-nested-images columns-default is-cropped wp-block-gallery-2 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"836\" height=\"630\" data-id=\"219\" src=\"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.06.51-PM.png\" alt=\"\" class=\"wp-image-219\" srcset=\"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.06.51-PM.png 836w, http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.06.51-PM-300x226.png 300w, http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.06.51-PM-768x579.png 768w\" sizes=\"auto, (max-width: 836px) 100vw, 836px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1120\" height=\"584\" data-id=\"225\" src=\"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.07.27-PM-edited.png\" alt=\"\" class=\"wp-image-225\" srcset=\"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.07.27-PM-edited.png 1120w, http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.07.27-PM-edited-300x156.png 300w, http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.07.27-PM-edited-1024x534.png 1024w, http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.07.27-PM-edited-768x400.png 768w\" sizes=\"auto, (max-width: 1120px) 100vw, 1120px\" \/><\/figure>\n<\/figure>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:100%\">\n<figure class=\"wp-block-gallery has-nested-images columns-default is-cropped wp-block-gallery-3 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"756\" height=\"657\" data-id=\"222\" src=\"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.09.48-PM.png\" alt=\"\" class=\"wp-image-222\" srcset=\"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.09.48-PM.png 756w, http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.09.48-PM-300x261.png 300w\" sizes=\"auto, (max-width: 756px) 100vw, 756px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1116\" height=\"667\" data-id=\"224\" src=\"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.10.18-PM-edited.png\" alt=\"\" class=\"wp-image-224\" srcset=\"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.10.18-PM-edited.png 1116w, http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.10.18-PM-edited-300x179.png 300w, http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.10.18-PM-edited-1024x612.png 1024w, http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.10.18-PM-edited-768x459.png 768w\" sizes=\"auto, (max-width: 1116px) 100vw, 1116px\" \/><\/figure>\n<figcaption class=\"blocks-gallery-caption\">The concentration of Pb in Fescue and Vetiver grass tissues before and after 1st EDDS Treatment <\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:100%\">\n<figure class=\"wp-block-gallery has-nested-images columns-default is-cropped wp-block-gallery-4 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"788\" height=\"549\" data-id=\"226\" src=\"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.09.14-PM-1.png\" alt=\"\" class=\"wp-image-226\" srcset=\"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.09.14-PM-1.png 788w, http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.09.14-PM-1-300x209.png 300w, http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.09.14-PM-1-768x535.png 768w\" sizes=\"auto, (max-width: 788px) 100vw, 788px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"794\" height=\"568\" data-id=\"231\" src=\"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.32.15-PM.png\" alt=\"\" class=\"wp-image-231\" srcset=\"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.32.15-PM.png 794w, http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.32.15-PM-300x215.png 300w, http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-content\/uploads\/2022\/02\/Screen-Shot-2022-02-25-at-9.32.15-PM-768x549.png 768w\" sizes=\"auto, (max-width: 794px) 100vw, 794px\" \/><\/figure>\n<figcaption class=\"blocks-gallery-caption\">The total concentration of lead leached from Vetiver and Fescue grass in Baltimore and San Antonio soils <\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The first chelating agent application was EDDS at 10 mmol\/kg. Leaching samples were analyzed before and after treatment of EDDS&hellip;<\/p>\n","protected":false},"author":5,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[10],"tags":[],"class_list":["post-208","post","type-post","status-publish","format-standard","hentry","category-phase-2-simulated-field-study"],"_links":{"self":[{"href":"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-json\/wp\/v2\/posts\/208","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-json\/wp\/v2\/comments?post=208"}],"version-history":[{"count":5,"href":"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-json\/wp\/v2\/posts\/208\/revisions"}],"predecessor-version":[{"id":477,"href":"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-json\/wp\/v2\/posts\/208\/revisions\/477"}],"wp:attachment":[{"href":"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-json\/wp\/v2\/media?parent=208"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-json\/wp\/v2\/categories?post=208"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/bio.sites.mtu.edu\/lead-remediation\/wp-json\/wp\/v2\/tags?post=208"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}